Journal: Clinical psychopharmacology and neuroscience : the official scientific journal of the Korean College of Neuropsychopharmacology

Article Title: Effects of Epothilone D on Social Defeat Stress-induced Changes in Microtubule-related and Endoplasmic Reticulum Stress Protein Expression.

doi: 10.9758/cpn.24.1212

Figure Lengend Snippet: Fig. 3. Results of two-way analysis of variance ANOVA for microtubule related proteins and endoplasmic reticulum stress proteins among the EpoD- and DMSO-treated groups in the PFC and HIP. CHOP, (C/EBP)-homologous protein; Con, control; DMSO, dimethylsulfoxide; GRP-78, 78-kDa glucoseregulated protein; HIP, hippocampus; MAP2, microtubule-associated protein-2; PFC, prefrontal cortex; p-STMN1, phospho-stathmin1; p-STMN2, phospho-stathmin2; STMN1, stathmin1; STMN2, stathmin2. *p ＜ 0.05, **p ＜ 0.01, ***p ＜ 0.001, ****p ＜ 0.0001 in PFC and HIP compared to each group.

Article Snippet: The membranes were then blocked with 5% skimmed milk and incubated at 4°C overnight with mouse monoclonal antibodies against tubulin (1:50,000; Sigma-Aldrich), acetylated tu- bulin (1:50,000; Sigma-Aldrich), tyrosinated tubulin (1: 1,000; Sigma), MAP2 (1:1,000; Sigma-Aldrich), and CHOP (1:1,000; Sigma-Aldrich); rabbit polyclonal antibodies against p-STMN1 (Ser16) (1:1,000; Cell Signaling Technology), phospho-stathmin1 serin 25 (p-STMN1 [Ser25]) (1:500; Invitrogen), phospho-stathmin1 serin 38 (p-STMN1 [Ser38]) (1:1,000; Cell Signaling Technology), and phospho-stathmin2 serin 73 (p-STMN2 [Ser73]) (1:1,000; Invitrogen); rabbit monoclonal antibodies against STMN1 (1:50,000; Abcam) and STMN2 (1:10,000; Abcam); and rabbit polyclonal antibodies against GRP-78 (1:1,000; Cell Signaling Technology) and -actin (1:1,000; Cell Signaling Technology).

Techniques: Control

Journal: Oncotarget

Article Title: Stathmin and phospho-stathmin protein signature is associated with survival outcomes of breast cancer patients

doi:

Figure Lengend Snippet: A. Representative IHC staining of high and low expression of STMN1 in the large (400×) and small images (100×). B. Representative IHC staining of high and low expression of multiple phosphor-sites (Ser-16, Ser-25, Ser38, Ser63) in the large (400×) and small images (100×). C. Kaplan-Meier analysis of DFS in the training set. D. Kaplan-Meier analysis of DFS in the validation set.

Article Snippet: For STMN1 and Ser38, TMAs were blocked with 10% normal goat serum for 1 h at room temperature (RT) and incubated in a humid chamber at 4°C overnight with polyclonal rabbit anti-human STMN1 antibody (Proteintech) or polyclonal rabbit anti-human STMN1 Ser38 (Cell Signaling Technologies) antibody diluted to 1:400 or 1:100, respectively.

Techniques: Immunohistochemistry, Expressing, Biomarker Discovery

Journal: Oncotarget

Article Title: Stathmin and phospho-stathmin protein signature is associated with survival outcomes of breast cancer patients

doi:

Figure Lengend Snippet: Univariate association of the STMN1-E/P model, clinicopathological characteristics, and single phospho-sites status with disease-free survival

Article Snippet: For STMN1 and Ser38, TMAs were blocked with 10% normal goat serum for 1 h at room temperature (RT) and incubated in a humid chamber at 4°C overnight with polyclonal rabbit anti-human STMN1 antibody (Proteintech) or polyclonal rabbit anti-human STMN1 Ser38 (Cell Signaling Technologies) antibody diluted to 1:400 or 1:100, respectively.

Techniques: Biomarker Discovery

Journal: Oncotarget

Article Title: Stathmin and phospho-stathmin protein signature is associated with survival outcomes of breast cancer patients

doi:

Figure Lengend Snippet: Data are shown as AUC (95% CI) or hazard ratios (95% CI). ROC = receiver operator characteristic. AUC = area under the curve. A. Comparisons of the prognostic accuracy by the STMN1-E/P model and TNM stage in the training set. B. DFS of patients with high- or low-risk scores in the training set. C. Comparisons of the prognostic accuracy by the STMN1-E/P model and TNM stage in the validation set. D. DFS of patients with high- or low-risk scores in the validation set. P values were calculated using the log-rank test.

Article Snippet: For STMN1 and Ser38, TMAs were blocked with 10% normal goat serum for 1 h at room temperature (RT) and incubated in a humid chamber at 4°C overnight with polyclonal rabbit anti-human STMN1 antibody (Proteintech) or polyclonal rabbit anti-human STMN1 Ser38 (Cell Signaling Technologies) antibody diluted to 1:400 or 1:100, respectively.

Techniques: Biomarker Discovery

Journal: Oncotarget

Article Title: Stathmin and phospho-stathmin protein signature is associated with survival outcomes of breast cancer patients

doi:

Figure Lengend Snippet: A. Comparisons of the prognostic accuracy by the STMN1-E/P/C model, STMN1-E/P model and TNM stage in the training set. B. DFS of patients with high- or low-risk scores according to the STMN1-E/P/C model in the training set. C. Comparisons of the prognostic accuracy by the STMN1-E/P/C model, STMN1-E/P model and TNM stage in the validation set. D. DFS of patients with high- or low-risk scores according to the STMN1-E/P/C model in the validation set. P values were calculated using the log-rank test. E. DFS of patients with luminal breast cancer. F. DFS of patients with HER2/neu subtype breast cancer. G. DFS of patients with TNBC subtype breast cancer.

Article Snippet: For STMN1 and Ser38, TMAs were blocked with 10% normal goat serum for 1 h at room temperature (RT) and incubated in a humid chamber at 4°C overnight with polyclonal rabbit anti-human STMN1 antibody (Proteintech) or polyclonal rabbit anti-human STMN1 Ser38 (Cell Signaling Technologies) antibody diluted to 1:400 or 1:100, respectively.

Techniques: Biomarker Discovery

Journal: bioRxiv

Article Title: Vector analysis of steerable mechanical tension across nuclear lamina

doi: 10.1101/462275

Figure Lengend Snippet: (A) MCF-7 cells were treated with ADM, H 2 O 2 , and CHX separately. The samples were stained for both β-actin and α-tubulin. The cells were also stained with DAPI to localize the nuclei. The images were captured through confocal laser microscopy after immunofluorescence staining (n = 10). (B) Intracellular nanoparticle size distribution in MCF-7 cells separately treated with ADM, H 2 O 2 , and CHX (n = 10). (C) MCF-7 cell intracellular osmotic pressure was measured via osmometery after the cells were exposed to ADM, H 2 O 2 , and CHX. (**: 0.001 < p < 0.05, ns: p > 0.05, Tukey-b test, n = 10). (D) P-cofilin, cofilin, actin, p-stathmin, stathmin, and tubulin levels in MCF-7 cells that had been treated with ADM, H 2 O 2 , or CHX (n = 6). (E) In the H 2 O 2 group, the ΔpNOP could be divided into two parts by comparing the total intensity of the protein nanoparticles, generated from MF and MT depolymerization, respectively (versus , line 1, row 2). Scale bar, 10 μm. All error bars represent SEM.

Article Snippet: The main antibodies and dilutions used were: rabbit anti-lamin B1 antibody (CST; 1:1000), rabbit anti-β-actin antibody (CST; 1:1000), mouse anti-tubulin-α antibody (T5168, Boster; 1:300), rabbit anti-vimentin antibody (CST; 1:1000), rabbit anti-GFP antibody (CST; 1:1000), mouse anti-phospho-cofilin (Ser24) (Bioss; 1:300), mouse anti-cofilin antibody (T5168, Boster; 1:300), rabbit anti-phospho-stathmin 1 (Ser38) (Bioss; 1:300), and mouse anti-stathmin 1 antibody (T5168, Boster; 1:300).

Techniques: Staining, Microscopy, Immunofluorescence, Generated

Journal: Hepatology Communications

Article Title: Stathmin 1 Induces Murine Hepatocyte Proliferation and Increased Liver Mass

doi: 10.1002/hep4.1447

Figure Lengend Snippet: Hepatic STMN1 mRNA and protein expression are increased in mouse and human liver injury. (A) Hepatic Stmn1 mRNA levels relative to uninjected control mice in mice treated for the indicated number of hours with CCl 4 by qRT‐PCR (* P < 0.01, ** P < 0.0002, compared to control; n = 3‐6). (B) Immunoblots of total hepatic protein from an untreated control mouse and CCl 4 ‐treated mice probed for total STMN1, Ser‐16‐ and Ser‐38‐phospho‐STMN1, and the loading control tubulin. (C) Relative levels of STMN1 mRNA in normal human livers and livers from patients with fulminant hepatic failure (* P < 0.007, compared to normal; n = 4). (D) Total human liver protein from the same livers immunoblotted for total and phosphorylated STMN1, Fos‐related antigen 1, and β‐actin as a loading control. Arrows in (B) and (D) indicate the respective protein bands and molecular weights in kilodaltons. Abbreviations: Con, control; FHF, fulminant hepatic failure; FRA1, Fos‐related antigen 1; h, hours; NL, normal liver; P 16 ‐stathmin, serine‐16 phosphorylated stathmin 1; P 38 ‐stathmin, serine‐38 phosphorylated stathmin 1.

Article Snippet: Total protein was isolated from mouse and human livers, as described., Membranes were probed with antibodies to β‐actin (#A5441; MilliporeSigma), caspase 3 (#9665; Cell Signaling, Beverly, MA), caspase 7 (#9492; Cell Signaling), cyclin A (#sc‐596; Santa Cruz Biotechnology, Santa Cruz, CA), cyclin B1 (#sc‐595; Santa Cruz Biotechnology), cyclin D1 (#MA1‐24750; Thermo Fisher Scientific), cyclin D2 (#sc‐593; Santa Cruz Biotechnology), cyclin D3 (#sc‐182; Santa Cruz Biotechnology), cyclin‐dependent kinase (CDK) 2 (#sc‐163; Santa Cruz Biotechnology), CDK4 (#sc‐260; Santa Cruz Biotechnology), E2F2 (#sc‐633; Santa Cruz Biotechnology), Fos‐related antigen 1 (#sc‐183; Santa Cruz Biotechnology), total STMN1 (#3352; Cell Signaling), phosphorylated (phospho)‐Serine (Ser)‐16 STMN1 (#3353; Cell Signaling), phospho‐Ser‐38 STMN1 (#3426; Cell Signaling), and tubulin (#9411; Cell Signaling).

Techniques: Expressing, Control, Quantitative RT-PCR, Western Blot